1
Question
After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule?
After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule?
Open in App
Solution
The correct option is C 16
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a technique used for the in vitro amplification of the DNA fragments or gene of interest to create multiple copies.
Steps involved in PCR
The steps involved in PCR are as follows:
- Denaturation - In this step separation of the two strands of double-stranded DNA is done by application of heat at 94 - 95°C.
- Annealing - This step involves the hybridization of the primers with specific sequences of each of the strands of the template DNA. Primers are small oligonucleotides up to 18 - 24 nucleotides long synthesised chemically. This process is carried out at 50 - 56°C.
- Extension - In this step, the Taq DNA polymerase synthesizes DNA by adding dNTPs (deoxyribonucleotides) to the free ends of the primers. The extension of DNA is carried out at 72°C. This helps in synthesizing complementary strands.
End products of PCR
In each step of PCR, the number of DNA molecules doubles. One DNA template forms 2 DNA molecules in PCR amplification. Thus, by the end of the 4th cycle of PCR, there are 16 DNA molecules formed.
Final Answer
c. 16
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a technique used for the in vitro amplification of the DNA fragments or gene of interest to create multiple copies.
Steps involved in PCR
The steps involved in PCR are as follows:
- Denaturation - In this step separation of the two strands of double-stranded DNA is done by application of heat at 94 - 95°C.
- Annealing - This step involves the hybridization of the primers with specific sequences of each of the strands of the template DNA. Primers are small oligonucleotides up to 18 - 24 nucleotides long synthesised chemically. This process is carried out at 50 - 56°C.
- Extension - In this step, the Taq DNA polymerase synthesizes DNA by adding dNTPs (deoxyribonucleotides) to the free ends of the primers. The extension of DNA is carried out at 72°C. This helps in synthesizing complementary strands.
In each step of PCR, the number of DNA molecules doubles. One DNA template forms 2 DNA molecules in PCR amplification. Thus, by the end of the 4th cycle of PCR, there are 16 DNA molecules formed.
Final Answer
c. 16
Suggest Corrections
0
Similar questions
View More
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
