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Question

After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule? 
  1. 4
  2. 32
  3. 16
  4. 8


Solution

The correct option is C 16
The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 4 cycles you have 16 copies, after 20 cycles you have about one million copies, etc.

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