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Question

After demonstration of totipotency, a botanist wishes to produce identical plants from different parts of angiosperm by tissue culture. He started with anther. The parts or tissues of anther which are likely to yield haploid embryos are:

A
Endothecium
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B
Sporogenous tissue
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C
Tapetal tissue
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D
Middle layer
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Solution

The correct option is B Sporogenous tissue

Totipotency in plants is the ability of a single cell to divide and differentiate into a complete plant. The tissue culture technique exploits this characteristic of plant cells and tissues for mass propagation of desired plants.

Almost all parts of the plants can be used for tissue culture. Diploid cells give rise to diploid embryos and haploid cells give rise to haploid embryos.

The anther is the part of stamen or the male reproductive part of a plant. Anther has 4 layers of walls enclosing the inner sporogenous tissue. The exterior epidermis, followed by endothecium and middle layer for protection. The innermost tapetal layer provides nutrition to the developing microspores.

Sporogenous tissues of anther consist of diploid microspore mother cells that undergo meiosis to form haploid microspores. These haploid microspores can give rise to haploid embryos in vitro.

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