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Question

AIDS can be detected by

A
RT-PCR
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B
ELISA
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C
Western blot test
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D
All of these
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Solution

The correct option is D All of these
AIDS can be detected by RT-PCR, ELISA and western blot test.

RT-PCR is a highly sensitive technique which is used for detection and quantification of RNA. In RT-PCR, the RNA is reverse transcribed into complementary DNA (cDNA), using reverse transcriptase, and then PCR is used to amplify the cDNA.

AIDS is caused by HIV, which is a retrovirus (viruses containing RNA as genetic material). RT-PCR is used for its detection as it amplifies even a single copy of RNA to produce millions of copies of cDNA. Thus the presence of even a tiny number of viruses can give a positive result, thereby allowing its early detection.

ELISA is a basic assay technique, known as enzyme-linked immunosorbent assay, that is carried out to detect and measure antibodies, antigens, hormones, peptides and proteins in the blood. ELISA works on the principle of antibody-antigen interaction. Antibodies are blood proteins produced in response to a specific antigen. ELISA helps to examine the presence of antibodies in the body, in case of certain infectious diseases like AIDS.

If a person tests positive for HIV on the ELISA test, the provider orders the Western blot test to confirm HIV infection.

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