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Question

Along with auxin, another hormone is used in tissue culture technique. It is

A
ABA
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B
Ethylene
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C
Cytokinin
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D
Gibberellin
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Solution

The correct option is C Cytokinin
Definition of tissue culture
Tissue culture is a technique where parts of a selected plant are aseptically transferred to an appropriate culture medium containing the required growth factors, leading to the formation of an entire plant.

Role of hormones in tissue culture
Phytohormones are chemical substances that are produced by the growing parts of the plant. These growth regulators need to be supplied in the medium for the division and differentiation of the explant during tissue culture. The main hormones that are needed initially in explant division and differentiation are auxins and cytokinins.

Gibberellin
Gibberellin is a plant hormone that induces the elongation of stem, leaf expansion, germination and flowering.

Cytokinin
Cytokinin is mainly responsible for cell division, induction of shoot formation, and growth of axillary shoots. In tissue culture, coconut milk that has zeatin, a naturally occurring cytokinin, is used as a source of the hormone.

Ethylene
Ethylene causes the ripening of fruits.

ABA
ABA( Abscisic acid) is the general inhibitor of growth and metabolism.


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