1
Question
Amount of DNA, RNA and protein simultaneously can be estimated by
Amount of DNA, RNA and protein simultaneously can be estimated by
Open in App
Solution
The correct option is B Autoradiography
In an autoradiography, radioactive isotopes (tritium, carbon-14, Phosphorous-32, Sulphur-35) are incorporated in intermediate or raw materials like carbon dioxide, uridine, thymidine, amino acids etc. for studying metabolic pathways including DNA to RNA to proteins pathway, otherwise known as Central Dogma of Molecular Biology.
The tissues are sectioned or squashed at intervals and then coated with photographic emulsion silver bromide in order to locate the product. Using this technique; Tritium in thymidine was employed by Taylor for studying chromosome replication. Tritium in uridine was used in studying RNA synthesis while tritium in amino acid like leucine is employed for study of protein synthesis.
In an autoradiography, radioactive isotopes (tritium, carbon-14, Phosphorous-32, Sulphur-35) are incorporated in intermediate or raw materials like carbon dioxide, uridine, thymidine, amino acids etc. for studying metabolic pathways including DNA to RNA to proteins pathway, otherwise known as Central Dogma of Molecular Biology.
The tissues are sectioned or squashed at intervals and then coated with photographic emulsion silver bromide in order to locate the product. Using this technique; Tritium in thymidine was employed by Taylor for studying chromosome replication. Tritium in uridine was used in studying RNA synthesis while tritium in amino acid like leucine is employed for study of protein synthesis.
Suggest Corrections
0
Similar questions
View More
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
