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Question
Ampicillin-sensitive bacteria are exposed to a plasmid containing the gene for resistance to the antibiotic ampicillin. They are then spread on two agar growth plates. Plate A contains growth medium only. Plate B contains growth medium and ampicillin.
Which plate might be expected to have cultures of ampicillin-resistant bacteria growing on it?
Ampicillin-sensitive bacteria are exposed to a plasmid containing the gene for resistance to the antibiotic ampicillin. They are then spread on two agar growth plates. Plate A contains growth medium only. Plate B contains growth medium and ampicillin.
Which plate might be expected to have cultures of ampicillin-resistant bacteria growing on it?
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Solution
The correct option is B Both plate A and plate B
When Ampicillin sensitive bacteria are exposed to plasmid containing the gene for resistance, all the sensitive strains will be now insensitive or resistant to Ampicillin, when grown on nutrient media with and without ampicillin, both will be having colonies of ampicillin resistant bacteria as the sensitive form has been converted to resistant form prior to segregating in growth plates.So, the correct answer is 'Both plate A and plate B'
When Ampicillin sensitive bacteria are exposed to plasmid containing the gene for resistance, all the sensitive strains will be now insensitive or resistant to Ampicillin, when grown on nutrient media with and without ampicillin, both will be having colonies of ampicillin resistant bacteria as the sensitive form has been converted to resistant form prior to segregating in growth plates.
So, the correct answer is 'Both plate A and plate B'
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