Focal length of the objective lens, fo=1.25cm
Focal length of the eyepiece, fe=5cm
Least distance of distinct vision, d=25cm
Angular magnification of the compound microscope = 30X
Total magnifying power of the compound microscope, m=30
The angular magnification of the eyepiece is given by the relation-
me=(1+d/fe)=6
The angular magnification of the objective lens (mo) is calculated as-
mome=m⇒mo=5
Now, mo=−vo/uo⇒vo=−5uo
Using lens formula,
1vo−1uo=1fo
Using above two equations, uo=−1.5cm and vo=7.5cm
So, The object should be placed 1.5 cm away from the objective lens to obtain the desired magnification.
Now, Image distance for the eyepiece is ve=−d=−25cm
Using lens formula,
1ve−1ue=1fe
ue=−4.17cm
Separation between the objective lens and the eyepiece, |ue|+|vo|=11.67cm
Therefore, the separation between the objective lens and the eyepiece should be 11.67cm.