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Question

An enzyme that cleaves DNA recognizes an 8 base-pair unique DNA sequence. The probable number of times this enzyme will cleave a 70 kilobase pair random DNA sequence is

A
1
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B
2
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C
3
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D
4
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Solution

The correct option is A 1
According to the question, the restriction sequence for the enzyme is a 8 base pair long sequence. If suppose, an arbitrary DNA sequence of 8 base pair as XXXXXXXX, where “X” can be any of four nucleotides (A/T/G/C); there are 4 possibilities for one “X” and the total sequence is 8 base pairs. For all 8 bases; the probability of having the given sequence is (4 x 4 x 4 x 4 x 4 x 4 x 4 x 4 = 65,536 base pairs) which means that the given restriction site occur every 65,536 base pairs. For a DNA segment of 70 kb; the possibility of having the given sequence = 70000/65,536 = around 1. The correct answer is A.

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