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Bacterial Synthesis of Insulin (Human)

Analyse the g...

Question

Analyse the given diagram which shows steps involved in the procedure of selecting transformed bacteria.
* In replica plating, a sterile pad is pressed on to plate M and then on the sterile agar in plate N.Identify the bacterial colony which has undergone transformation?

A

Colony 5

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B

Colony 2

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C

Colony 4

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D

Colony 3

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Solution

The correct option is B Colony 4
Bacterial colony 4 fails to grow on agar containing tetracycline and ampicillin and must, therefore, contain the inactive tetracycline resistance gene with the inserted DNA. These bacteria are transformed, hence should be cloned and amplified.
So, the correct option is 'Option C'.

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Bacterial Synthesis of Insulin (Human)

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