MyQuestionIcon

1

You visited us 1 times! Enjoying our articles? Unlock Full Access!

Bacterial Synthesis of Insulin (Human)

Analyse the g...

Question

Analyse the given diagram which shows steps involved in the procedure of selecting transformed bacteria.
* In replica plating, a sterile pad is pressed on to plate M and then on the sterile agar in plate N.Identify the bacterial colony which has undergone transformation?

A

Colony 5

No worries! We‘ve got your back. Try BYJU‘S free classes today!

B

Colony 2

No worries! We‘ve got your back. Try BYJU‘S free classes today!

C

Colony 4

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

D

Colony 3

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is B Colony 4
Bacterial colony 4 fails to grow on agar containing tetracycline and ampicillin and must, therefore, contain the inactive tetracycline resistance gene with the inserted DNA. These bacteria are transformed, hence should be cloned and amplified.
So, the correct option is 'Option C'.

Suggest Corrections

thumbs-up

0

Similar questions

10^{9}

bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37

^{0}

10

bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by

Q. In the given diagram which of the following plates has maximum bacterial growth?

Q. A gene is inserted within the BamH I cloning site in pBR322. Some plasmids take up the desired gene and form recombinant DNA while some fail to take it up. This mixture of plasmids is introduced into bacteria which are first grown on a medium ‘A’ containing only ampicillin. It is then replica plated on to another medium ‘B’ in which both ampicillin and tetracycline are present. Given below are the illustrations of colonies found on the two culture media. Analyse them and identify the bacterial colonies of recombinants.

Q. Ampicillin-sensitive bacteria are exposed to a plasmid containing the gene for resistance to the antibiotic ampicillin. They are then spread on two agar growth plates. Plate A contains growth medium only. Plate B contains growth medium and ampicillin.
Which plate might be expected to have cultures of ampicillin-resistant bacteria growing on it?

Q. Assertion: Charges are given to plates of two plane parallel plate capacitors

C_{1}

C_{2}

C_{2} = 2 C_{1}

)as shown in figure. Then the key

K

is pressed to complete the circuit. Finally the net charge on upper plate and net charge the circuit. Finally the net charge on upper plate and net charge on lower plate of capacitor

C

is positive.
Reason : In a parallel plate capacitor both plates always carry equal and opposite charge.

View More

Join BYJU'S Learning Program

similar_icon

Related Videos

lock

Applications in Medicine - Insulin Case Study

BIOLOGY

Watch in App

explore_more_icon

Explore more

Bacterial Synthesis of Insulin (Human)

Standard XII Biology

Join BYJU'S Learning Program

CrossIcon