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Androgenic haploid plants were produced by anther culture first time by
Androgenic haploid plants were produced by anther culture first time by
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Solution
The correct option is C Sipra Guha and P. Maheswari
In vitro culture of anthers was first undertaken by a Japanese cytologist Shimakura (1934) with the objective of understanding the physiology of meiosis. Guha and Maheshwari (1964) also cultured mature anthers of Datura innoxia with a similar aim, but accidentally made a more significant observation. They noticed that on nutrient medium containing kinetin, coconut milk or grape juice numerous embryos appeared from the inside of anthers which eventually developed into plantlets. In 1966, these workers confirmed the origin of the embryos from pollen grains.
In vitro culture of anthers was first undertaken by a Japanese cytologist Shimakura (1934) with the objective of understanding the physiology of meiosis. Guha and Maheshwari (1964) also cultured mature anthers of Datura innoxia with a similar aim, but accidentally made a more significant observation. They noticed that on nutrient medium containing kinetin, coconut milk or grape juice numerous embryos appeared from the inside of anthers which eventually developed into plantlets. In 1966, these workers confirmed the origin of the embryos from pollen grains.
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