Annaya wanted to isolate and study organelles involved in packaging and transporting of proteins to various locations in a cell. Her colleague had carried out the following experiment: She ground a piece of spinach leaves and carried out differential centrifugation. A scheme of the protocol she followed along with the centrifugation speed (in g) at every step is given.
The fraction that would give Annaya the purified fraction of the organelle of her interest would be?

1

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

2

No worries! We‘ve got your back. Try BYJU‘S free classes today!

3

No worries! We‘ve got your back. Try BYJU‘S free classes today!

4

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is A $1$
although biochemical analysis requires disruption of the anatomy of the cell, gentle fractionation techniques have been devised to separate the various cell components while preserving their individual functions. Just as a tissue can be separated into its living constituent cell types, so the cell can be separated into its functioning organelles and macromolecules.
So, the correct option is '1'.

Suggest Corrections

Similar questions

Q. A piece of liver tissue is homogenized in a laboratory and the homogenate is subjected to sequential centrifugation at increasing speeds (expressed in xg). Centrifugation is used to separate sub-cellular components based on size and density. The steps carried out in the experiment are shown.
Sediments

1, 2

and

3

would most likely contain.

A student carrying out cell fractionation of plant tissue forgot to label her tubes. The contents of one tube when studied showed organelles bounded by single membrane with acidic internal components. These organelles could be:

Q. Which cellular organelle would be concentrated at the top of the centrifuged mixture after centrifugation of cells containing solution?

Rita wants to start a business of garments, further, she plans to set up her business in the rural area so that she can get cheap raw materials and workers easily, she also wants to donate 10% of her profit to the primary school which is located near her business.

(a) What possible risks she can face in carrying out this business.

(b) Give two values which she is delivering to the society.

Rita carried out an experiment, she took two transparent plastic jars and labeled them as Jar 1 and Jar 2. In Jar 1 she placed a cockroach, pieces of bread and water. In Jar 2 she placed a cockroach, pieces of bread but no water. She then covered both the jars with a mosquito net. What will be her observations after a few days?

Join BYJU'S Learning Program