In this transport system ATP is used to pump molecules AGAINST/UP the concentration gradient.
Transportation happens from lower concentration solution to higher concentration solution.
It requires cellular energy as the transfer is against the concentration.
Passive transport:
In this transport system, molecule transportation is done down the concentration.
It goes from a high to a low concentration to keep the cells in balance.
It does not need any energy to transfer molecules.
Carrier proteins:
They are essential proteins that carry chemicals across the membrane/cell in both the direction against and down the concentration gradient.
It requires energy while transferring substances against the concentration gradient.
Carrier proteins are both active and passive.
Example: sodium-potassium pump, glucose-sodium cotransport, etc.
Valinomycin acts as a passive carrier protein that increases the permeability of potassium ions down its gradient from higher concentration to lower concentration without the use of energy.
The sodium-potassium ATPase pump transports molecules actively using ATPs. It moves two sodium ions out of the cell for every three potassium ions moved inside.