In photosynthesis, light-independent reactions are known as dark reactions. This process does not depend directly on the presence of light but is dependent on the products of light reaction i.e., ATP and NADPH. The dark reactions occur through Calvin cycle. They take place in the stromal matrix of the chloroplast. The enzymes that catalyse the dark reaction of carbon fixation are present outside the thylakoids. These enzymes are present in the stromal matrix of chloroplasts.