5
Question
Arrange the following steps in the correct order for the production of the transgenic mice
I) Establishment of transgenic mice strain
II) Foreign DNA is injected into the pronuclei
III) Mice are allowed to mate and fertilised egg from the female is procured
IV) Transfer of embryos to the female mouse
V) Offspring mice are tested for the presence of transgene
VI) Breeding of offspring mice carrying the transgene
Arrange the following steps in the correct order for the production of the transgenic mice
I) Establishment of transgenic mice strain
II) Foreign DNA is injected into the pronuclei
III) Mice are allowed to mate and fertilised egg from the female is procured
IV) Transfer of embryos to the female mouse
V) Offspring mice are tested for the presence of transgene
VI) Breeding of offspring mice carrying the transgene
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Solution
The correct option is D III, II, IV, V, VI, I
Transgenic mice have their DNA manipulated to possess and express an extra (foreign) gene. The following are the steps which are followed for creation of transgenic mice.
A. Mice are allowed to mate and immediately the fertilised egg from the female is procured (The fertilised egg is removed from the female just before the fusion of the male and female pronuclei). (III)
B. The desired transgene (foreign DNA) is microinjected (technique of direct transfer of gene using micropipette) into the pronucleus of the sperm. A pronucleus is the haploid nucleus of either the male or the female gamete, both present in the fertilised egg prior to their fusion. (II)
C. After microinjection, the fertilised eggs are allowed to develop into embryos. The embryos are implanted in the uterus of the pseudopregnant female mouse (a pseudopregnant female mouse is the one which is allowed to mate with an infertile male mouse that brings about hormone-induced uterine changes to make the uterus suitable for implantation). (IV)
D. Mice offspring obtained are tested for the presence of transgene. Moreover, since the DNA was injected at the one-celled stage, these mice usually carry the foreign DNA in every cell, including their reproductive cells, and therefore can pass the foreign DNA to their progeny. (V)
E. Interbreeding of offspring mice which carries the transgene is performed to establish a transgenic line. (VI)
F. A strain of mice that carries the foreign gene (s) is created. (I)
Transgenic mice have their DNA manipulated to possess and express an extra (foreign) gene. The following are the steps which are followed for creation of transgenic mice.
A. Mice are allowed to mate and immediately the fertilised egg from the female is procured (The fertilised egg is removed from the female just before the fusion of the male and female pronuclei). (III)
B. The desired transgene (foreign DNA) is microinjected (technique of direct transfer of gene using micropipette) into the pronucleus of the sperm. A pronucleus is the haploid nucleus of either the male or the female gamete, both present in the fertilised egg prior to their fusion. (II)
C. After microinjection, the fertilised eggs are allowed to develop into embryos. The embryos are implanted in the uterus of the pseudopregnant female mouse (a pseudopregnant female mouse is the one which is allowed to mate with an infertile male mouse that brings about hormone-induced uterine changes to make the uterus suitable for implantation). (IV)
D. Mice offspring obtained are tested for the presence of transgene. Moreover, since the DNA was injected at the one-celled stage, these mice usually carry the foreign DNA in every cell, including their reproductive cells, and therefore can pass the foreign DNA to their progeny. (V)
E. Interbreeding of offspring mice which carries the transgene is performed to establish a transgenic line. (VI)
F. A strain of mice that carries the foreign gene (s) is created. (I)
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