Question

Arrange the following steps of ELISA in sequential order.
a) Addition of substrate in the linked enzyme.

b) Attachment of antibody conjugated with an enzyme.
c) Coating of virus sample on the surface.

• A. a), c), b)
• B. a), b), c)
• C. c), b), a)
• D. c), a), b)

Answer 1

Answer: (C)

c), b), a)

The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The specific antigen or virus particle is coated to the wells of the ELISA plate. If there is specific (primary) antibody in the sample, they will bind to the antigen which is coated. The secondary antibody attached to the enzyme binds to the primary antibody. The chromogenic substrate is added which is acted upon by the enzyme and the colour is produced which can be read and detected by the ELISA reader.

Thus, the correct answer is option C.