Assertion(A) : Fine structure of the objects can be observed by transmission electron microscope Reason(R) : Study of living cells cannot be done through TEM, because the high voltage it employs, kills the cells
A
Both assertion and reason are true and the reason is the correct explanation of the assertion
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B
Both assertion and reason are true but the reason is not the correct explanation
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C
The assertion is true but the reason is false
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D
Both assertion and reason are false
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Solution
The correct option is B Both assertion and reason are true but the reason is not the correct explanation According to assertion statement fine structures can be observed by Transmission electron microscope (TEM).
The reason statement, study of live cells is not possible through TEM. The reason being is that the specimen will be under high pressure in Electron microscope chamber. And also sample is prepared by covalent crosslinking of gluteraldehyde and osimum tetraoxide later by dehydrating and embedding in plastic.
Only the fixed specimen can be visualized clearly using TEM