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Screening of Recombinant Cell

Assertion :E....

Question

Assertion :E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for BamHI is present in $t e t^{R}$ region of pBR322.

Both Assertion and Reason are correct and Reason is the correct explanation for Assertion

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Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion

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Assertion is correct but Reason is incorrect

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Both Assertion and Reason are incorrect

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Solution

The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
pBR322 has recognition sites for several commonly used restriction enzymes. Recognition site for BamHI is present in $t e t^{r}$ region i.e., region responsible for tetracycline resistance. When an insert is added at the BamHI recognition site the gene for tetracycline resistance becomes non-functional and the recombinant bacteria with plasmid pBR322 that has DNA insert at BamHI lose tetracycline resistance.
So, the correct answer is 'Both Assertion and Reason are correct and Reason is the correct explanation for Assertion'.

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