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Question

Assertion :ELISA test is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins, glycoproteins, etc.) on it. Reason: The pathogen antibody to be identified is immibilised on the surface of specially constructed ELISA plares and is then tested.

A
Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
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B
Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
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C
Assertion is correct but Reason is incorrect
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D
Both Assertion and Reason are incorrect
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Solution

The correct option is D Both Assertion and Reason are incorrect
ELSA (enzymes linked immuno-sorbant assey) is a technique based on the principle of antigen-antibody interactions. Pathogens have specific antigens on their surface like protein, glycoproteins, etc. Thus, their infection can be detected by using the antibodies specific to bind with those antigens. In process antigens are immobilised on ELISA plates. Then antibody specific to the antigen is added and allowed to react with the adsorbed antigen. A complex procedure is followed.
So, the correct answer is 'Both Assertion and Reason are incorrect'.

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