Assertion: Gold or tungsten microparticles coated with DNA are used in biolistics.
Reason: Gold and Tungsten are inert.

Both the assertion and reason are true and the reason is the correct explanation of assertion

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Both the assertion and reason are true and reason is not the correct explanation of assertion

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Assertion is true but the reason is false

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Both the assertion and reason are false

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Solution

The correct option is A Both the assertion and reason are true and the reason is the correct explanation of assertion
The process of transfer of exogenous DNA into host cells is known as transformation.
Biolistics is one of the methods of transformation. It is one of the methods of direct gene delivery into host cells. It is also known as a gene gun. In this method, plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.

Gold or tungsten is used as they are fairly inert/ non-reactive in nature. Hence, they will not be toxic to the target cells.

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The technique in which a foreign DNA is coated on the surface of tungsten or gold particles and shot into the target cells is known as:

	Column I		Column II
(i)	Electroporation	(p)	Recombinant DNA is coated with lipid
(ii)	Micro-injection	(q)	Tiny high velocity particles of tungsten or gold are coated with recombinant DNA
(iii)	Lipofection	(r)	Cells are exposed to rapid pulse of high voltage
(iv)	Particle Bombardments	(s)	Directly injected to the nucleus of animal cell through micro

For transformation, microparticles coated with DNA to be bombarded with Gene gun are made up of

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