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Question
Assertion :Gram-negative bacteria do not retain the stain, when washed with alcohol. Reason: The outer face of the outer membrane of Gram - negative bacteria contains lipoplysaccharides, a part of which is integrated into the membrance lipids.
Assertion :Gram-negative bacteria do not retain the stain, when washed with alcohol. Reason: The outer face of the outer membrane of Gram - negative bacteria contains lipoplysaccharides, a part of which is integrated into the membrance lipids.
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Solution
The correct option is B Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
Gram-negative bacteria do not retain the stain because when alcohol is added, it dissolves the lipids found on the outer membrane which results in leaking of crystal violet stain out through the peptidoglycan layer which is thin.Both assertion and reason are correct statements. But the reason given does not actually explain the real reason why the stain is not retained.Therefore the right answer is option B, Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
Gram-negative bacteria do not retain the stain because when alcohol is added, it dissolves the lipids found on the outer membrane which results in leaking of crystal violet stain out through the peptidoglycan layer which is thin.
Both assertion and reason are correct statements. But the reason given does not actually explain the real reason why the stain is not retained.
Therefore the right answer is option B, Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
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