Assertion :HAT medium is used for selection of fused (hybridoma) cells. Reason: Unfused myeloma cells cannot grow because they lack HGPRT and thus cannot replicate their DNA.

Both Assertion and Reason are correct and Reason is the correct explanation for Assertion

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Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion

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Assertion is correct but Reason is incorrect

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Both Assertion and Reason are incorrect

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Solution

The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
HAT medium is used for selecting the hybridoma cells produced by fusion. HAT medium has aminopterin that prevents the DNA synthesis and growth of unfused myeloma cells. Myeloma cells have HGPRT from the spleen cells that helps them to synthesize DNA by help of salvage pathway.
So the correct answer is "Both assertion and reason are correct and reason is the correct explanation for assertion".

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