Question

Assertion :Human insulin can be produced into bacterial cells using biotecnnology. Reason: To produce human insulin the A, B and C polypeptides of the human insulin are produced seperately in the bacterial cells, extracted and combined by creating disulphide bonds.

• A. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
• B. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
• C. Assertion is correct but Reason is incorrect
• D. Both Assertion and Reason are incorrect

Answer 1

The correct option is C Assertion is correct but Reason is incorrect

• Management of adults onset diabetes is possible by taking insulin at regular time intervals but insulin from other animals could elicit an immune response in the body.
• Biotechnology has helped to overcome this problem. Transgenic bacteria have been produced that translate the constituting polypeptide chains of human insulin. These polypeptides can be extracted from the bacterial cells and combined by creating disulphide bonds to produce human insulin.
• In human insulin is produced as a prohormone with three polypeptides A, B and C. After processing, the C peptide is removed and mature insulin is formed. When transgenic bacteria are used, instead of producing extra stretch of C peptide, only A and B polypeptides are produced and then linked to produce mature insulin directly.