Question

Assertion: In pronuclear microinjection, transgene is injected into the male pronucleus.
Reason: In this method, a holding pipette is used to hold the fertilised oocyte in place.

• A. Both assertion and reason are true
• B. Only assertion statement is true
• C. Only reason statement is true
• D. Both assertion and reason statement are false

Answer 1

The correct option is A Both assertion and reason are true

For mouse transgenesis, DNA can be introduced in the mice by pronuclear microinjection of DNA into the male pronucleus. A holding pipette is used that helps to hold the fertilised oocyte in place during microinjection. The microinjection pipette is used to pierce the oocyte and then the male pronucles, after which the DNA is introduced directly into the pronucleus.