Assertion: : Insertion of recombinant DNA within the coding sequence of beta-galactosidase results in colourless colonies.

Reason: Presence of insert results in inactivation of enzyme beta-galactosidase known as insertional inactivation.

If both assertion and reason are true and reason is the correct explanation of assertion

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If both assertion and reason are true but reason is not the correct explanation of assertion

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If assertion is true but reason is false

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If both assertion and reason are false

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Solution

The correct option is A If both assertion and reason are true and reason is the correct explanation of assertion
The plasmid in the bacteria, lacking an insert produces blue coloured colonies, while those plasmids with an insert do not produce any colour due to insertional inactivation of the enzyme, beta-galactosidase. Beta - galactosidase, when intact, cleaves X-gal to release a blue coloured product.

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Assertion: : Insertion of recombinant DNA within the coding sequence of beta-galactosidase results in colourless colonies. Reason: Presence of insert results in inactivation of enzyme beta-galactosidase known as insertional inactivation.

Assertion: : Insertion of recombinant DNA within the coding sequence of beta-galactosidase results in colourless colonies.

Reason: Presence of insert results in inactivation of enzyme beta-galactosidase known as insertional inactivation.