Assertion: Isolated protoplasts are used for somatic hybridisation
Reason: Callus culture does not allow variation

Both assertion and reason are true and reason is the correct explanation of assertion

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Both assertion and reason are true but reason is not the correct explanation of assertion

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Assertion is true but reason is false

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Both assertion and reason are false

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Solution

The correct option is B Both assertion and reason are true but reason is not the correct explanation of assertion
Somatic hybridisation involves the fusion of isolated protoplasts of two different cells, each having a desirable characteristic. This can be then used to grow a new variety of plant.

Callus is an unorganised proliferative mass of cells produced from isolated cells, tissues or organs. Hence it doesn't allow considerable variations. It is grown on artificial nutrient medium under controlled conditions.

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Assertion: Isolated protoplasts are used for somatic hybridisation Reason: Callus culture does not allow variation

Assertion: Isolated protoplasts are used for somatic hybridisation
Reason: Callus culture does not allow variation