Question

Assertion :One application of genetic engineering is the production of human insulin by microbes. Reason: Gene for production of human insulin can be transferred to Escherichia coli by recombinant DNA technique.

• A. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
• B. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
• C. Assertion is correct but Reason is incorrect.
• D. Assertion is incorrect but Reason is correct.

Answer 1

The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.

In the production of human insulin by bacterium, Escherichia coli, the human insulin gene is incorporated into the genetic material of this microorganism. The mutant bacteria multiply forming lineages of insulin-producing bacteria. To create a mutant bacteria capable of producing insulin, a plasmid is submitted to the action of restriction endonucleases specialized in cutting DNA fragments. The once circular plasmid is open by the restriction enzyme. The same enzyme is used to cut a human DNA molecule containing the insulin gene. The piece of human DNA containing the insulin gene then has its extremities bound to the plasmid with the help of DNA ligases. The recombinant plasmid containing the human insulin gene is then inserted into the bacterium, Escherichia coli for the insulin production.