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Question
Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason: In pBR322 alien DNA is ligated generally in the area of Bam HI site of tetracyline resistance gene.
Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason: In pBR322 alien DNA is ligated generally in the area of Bam HI site of tetracyline resistance gene.
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Solution
The correct option is B Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
The restriction enzyme has specific recognition sites in the plasmid vector which can be used for the insertion of the new gene. This is the site where a cut can be made and the new foreign gene can be introduced. Only one site for each restriction enzyme is present in the plasmid vector like in the pBR322, there is a BamHI site and tetracycline resistance gene which can be used for the introduction of the gene. This is a selectable marker used to check whether the cells are recombinant.Thus, the correct answer is option B.
The restriction enzyme has specific recognition sites in the plasmid vector which can be used for the insertion of the new gene. This is the site where a cut can be made and the new foreign gene can be introduced. Only one site for each restriction enzyme is present in the plasmid vector like in the pBR322, there is a BamHI site and tetracycline resistance gene which can be used for the introduction of the gene. This is a selectable marker used to check whether the cells are recombinant.
Thus, the correct answer is option B.
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