Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzymes. Reason: In $p B R 322$ alien $D N A$ is ligated generally in the area of $B a m - H I$ site of tetracycline resistance gene.

Both Assertion and Reason are correct and Reason is the correct explanation for Assertion

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Assertion is correct but Reason is incorrect

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Both Assertion and Reason are incorrect

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
Recognition site should be preferably single and responsive to commonly used restriction enzymes so it can be easily fragmented out. It should be single because we only want a cut at the required gene.
In pBR322 alien DNA is ligated in the area of Bam - HI site of tetracycline resistance gene. Hence there is only one recognition site where the cut is made.
So the correct answer is ' Both Assertion and Reason are correct and Reason is the correct explaination for the Assertion'.

Suggest Corrections

Similar questions

H_{2}

H e

(1 + \frac{P b}{R T})

H_{2}

H e

Assertion – The non-fusible impurities present in iron ore are removed by $C a C O_{3}$ .

Reason: $C a C O_{3}$ which decomposes into $C a O$ and $C O_{2}$ . The $C a O$ formed reacts with $S i O_{2}$ to remove silica

{SiO}_{2}

{CaCO}_{3}

CaO

{CaCO}_{3}

{SiO}_{2}

ASSERTION: n-Pentane and n-Hexane belong to a homologous series.

REASON: n-Pentane and n-Hexane belong to the same class of hydrocarbons and they differ by a - $C H_{2}$ - group.

Join BYJU'S Learning Program