Question

Assertion: Sticky ends forms hydrogen bonds with their complementary cut counterparts.
Reason: In recombinant DNA technology, sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes.

• A. Both assertion and reason are true and the reason is the correct explanation of the assertion
• B. Both assertion and reason are true but the reason is not the correct explanation of the assertion
• C. The assertion is true but the reason is false
• D. Both the assertion and reason are false

Answer 1

The correct option is B Both assertion and reason are true but the reason is not the correct explanation of the assertion

Sticky ends are produced by restriction enzymes when the cleavage point lies away from the axis of symmetry within the recognition site. This generates overhangs either at 5’ or 3’ ends. They help in the formation of hydrogen bonds with their complementary cut counterparts.
In recombinant DNA technology sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes. It is so because sticky ends increase the chance of recombinant DNA formation by providing an overhang, which is not the case with the blunt end producing restriction enzymes.
Thus, both assertion and reason are correct but the reason is not the correct explanation of the assertion.