Assertion :The matrix used in gel electrophoresis should have controllable pore size. Reason: Agarose concentration can be changed to I change pore sizes.
A
Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
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B
Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
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C
Assertion is correct but Reason is incorrect
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D
Both Assertion and Reason are incorrect
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Solution
The correct option is B Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion Agarose is a polysaccharide obtained from red algae. Agarose dissolves in hot water and when it is cooled, it forms a gel. Pore size depends upon agarose concentration. In general, a 1% (w/v) gel will have a pore size of 150 nm while a 0.16% gel has a pore size of 500 nm.