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Assertion :The RNAi can be introduced in an organism by insertion of gene encoding complementary RNA only Reason: these are no methods by which in vitro synthesized complementary RNA can be inserted in an organism to induce RNAi (RNA interference)
Assertion :The RNAi can be introduced in an organism by insertion of gene encoding complementary RNA only Reason: these are no methods by which in vitro synthesized complementary RNA can be inserted in an organism to induce RNAi (RNA interference)
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Solution
The correct option is C Assertion is correct but Reason is incorrect
Double stranded RNA mediated interference (RNAi) is a simple and rapid method of silencing gene expression. The silencing of a gene is a consequence of degradation of RNA into short RNAs that activate ribonucleases to target homologous mRNA. The RNAi can be introduced in an organism by insertion of gene encoding complementary RNA only.The two approaches most commonly used to silence a given gene in the lab through RNAi are: 1. The introduction into cells of in vitro synthesized small interfering RNA (siRNA).2. The use of DNA templates known as short hairpin RNA (shRNA) that host cells will use to generate the siRNAs.So the correct answer is 'Assertion is correct but Reason is incorrect'.
Double stranded RNA mediated interference (RNAi) is a simple and rapid method of silencing gene expression. The silencing of a gene is a consequence of degradation of RNA into short RNAs that activate ribonucleases to target homologous mRNA. The RNAi can be introduced in an organism by insertion of gene encoding complementary RNA only.
The two approaches most commonly used to silence a given gene in the lab through RNAi are:
1. The introduction into cells of in vitro synthesized small interfering RNA (siRNA).
2. The use of DNA templates known as short hairpin RNA (shRNA) that host cells will use to generate the siRNAs.
So the correct answer is 'Assertion is correct but Reason is incorrect'.
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