Assuming human pupil to have a radius r = 0.25 cm or diameter d = 2r = 0.5 cm
The wavelength of light is λ = 500 nm = 5 × 10−7 m.
sin θ=1.22λd
sin θ = 1.22×5×10−70.5×10−2 = 1.22 × 10−4
The distance of comfortable viewing is D = 25 cm = 0.25 m.
Let x be the minimum separation between two objects that the human eye can resolve.
sin θ = xD
D sin θ = 0.25 × 1.22 × 10−4 = 3 × 10−5 m = 30 μm.