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Question

At optimum temperature and pH, time required to reach maximum velocity of a reaction is 8 seconds, then what will be the time when substrate concentration is Km?
886964_2aad0e38d28b4eb59e74524be073d6c4.bmp

A
4 sec
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B
16 sec
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C
1 sec
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D
2 sec
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Solution

The correct option is A 4 sec
Michelis-menton reaction, calculates the rates of enzyme controlled reactions.
Where, V is the rate of reaction, Vmax is the maximum rate achieved by the system
S is the substrate concentration
Km is the substrate concentration where reaction rate is half of Vmax
Therefore the time taken for substrate concentration to reach Km is 4secs
So, the correct answer is '4 sec'

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