Question

At which temperature a DNA molecule is denatured by heat in PCR

• A. 70${}^o$ - 80${}^o$C
• B. 90${}^o$ - 95${}^o$C
• C. 42${}^o$C
• D. 50${}^o$ - 65${}^o$C

Answer 1

The correct option is B 90${}^o$ - 95${}^o$C

Denaturation: The reaction temperature is increased to 95 degree centigrade, which melts (disrupts the hydrogen bond between complementary bases) all dsDNA into single-stranded DNA (ssDNA).

So, option B is the correct option.