The correct option is A detect the presence of auxin in the Avena coleoptile tip.
As per the experiment conducted by Charles Darwin and his son Francis Darwin on the coleoptiles of canary grass, they observed the growth of the tips by bending towards the light source i.e., phototropism. F.W. Went isolated auxins from the tips of the coleoptile of Avena (Oats) seedlings, which bent towards light. The experiment conducted by Went is the Avena coleoptile test which is a bioassay for the detection of auxin.