Question

Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artificial chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the increasing cloning capacity?

• A. BAC, cosmid, phage, plasmid, YAC
• B. YAC, BAC, cosmid, phage, plasmid
• C. Plasmid, phage, cosmid, BAC, YAC
• D. Plasmid, Cosmid, phage, BAC, YAC

Answer 1

Answer: (C)

Plasmid, phage, cosmid, BAC, YAC

Plasmid - generally up to 15 Kb long DNA fragments
Phage - up to 23 Kb long DNA fragments
Cosmid - up to 45 Kb long DNA fragments
BAC - up to 300-350 Kb long DNA fragments
YAC - up to 1 Mb long DNA fragments