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Question

Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artificial chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the increasing cloning capacity?


A
BAC, cosmid, phage, plasmid, YAC
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B
YAC, BAC, cosmid, phage, plasmid
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C
Plasmid, phage, cosmid, BAC, YAC
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D
Plasmid, Cosmid, phage, BAC, YAC
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Solution

The correct option is D Plasmid, phage, cosmid, BAC, YAC
Plasmid - generally up to 15 Kb long DNA fragments 
Phage - up to 23 Kb long DNA fragments
Cosmid - up to 45 Kb long DNA fragments
BAC - up to 300-350 Kb long DNA fragments
YAC - up to 1 Mb long DNA fragments

Biology

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