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Central Dogma of Molecular Biology

Bacterial DNA...

Question

Bacterial DNA protects its own DNA from being cut up by the restriction enzymes present in the cell because _____________ .

A

bacterial DNA has no recongition sequences

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B

some of the nucleotides have caps of methyl group

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C

recognition sequences are covered with proteins

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D

only foriegn DNA can activate the restriction enzymes

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Solution

The correct option is B some of the nucleotides have caps of methyl group
Restriction enzymes provide a protective mechanism to the bacterial cell by cutting the foriegn DNA but the bacterial DNA is also prone to the action of these enzymes. Cutting its own DNA is prevented because some of the nucleotides of recognition sites have caps of methyl groups. They are methylated. So the enzymes do not have access to the recognition sites for their action.

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Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

Q. Read the following statements and select the correct ones.

(i) The same kind of sticky ends are produced when DNA has been cut by different restriction enzymes.

(ii) Exonucleases make cuts at specific positions within the DNA.

(iii) Hind II was the first restriction endonuclease to be isolated.

(iv) A bacteriophage can replicate within bacterial cells by integrating its DNA with bacterial DNA.

(v) The presence of more than one recognition site for an enzyme within the vector complicates the gene cloning.

Q. Bacteria uses restriction endonuclease to protect itself from viral attack. The bacterial DNA does not get degraded by its own enzyme because

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

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