Question

BamH I site in present at

• A. $rop$
• B. $am{p}^{R}gene$
• C. $te{t}^{R}gene$
• D. $Ori$

Answer 1

The correct option is C $te{t}^{R}gene$

pBR322
pBR322 is the most common plasmid vector. It is named after Bolivar and Rodriguez.

Features of pBR 322
Common features of pBR322 are as follows:
● Restriction sites - It has multiple restriction sites for different restriction enzymes such as Pvu II, Pvu I, BamH I, EcoR I, Hind III, Pst I, Sal I, Cla I, etc.
● Rop - It is a specific base sequence that codes for the rop protein involved in the replication of the plasmid.
● Selectable markers - It has two selectable markers, one for ampicillin resistance ampR and one for tetracycline resistance tetR.
● Ori - It has an ori site or origin of replication site. Any piece of DNA when linked to this sequence can be made to replicate within the host cells.

Pictorial representation of pBR322:


Figure : pBR322

Restriction sites at selectable markers
The restriction sites present at the selectable marker gene region on pBR322 are as follows:
● tetR - BamH I and Sal I
● ampR - Pvu I and Pst 1

Final answer
(B) $te{t}^{R}gene$