Question

Binding of a specific protein on regulatory DNA sequences can be studied by means of

• A. Ultracentrifuge.
• B. Electron microscope.
• C. Light microscope.
• D. X-ray crystallography.

Answer 1

The correct option is D X-ray crystallography.

X-ray crystallography is a technique to study the structural details of crystallizable proteins and nucleic acids. X-ray microscope developed by Kirkpatrick is useful in studying detailed structures of chemicals present in solid-state, e.g., hemoglobin, insulin, DNA, RNA, etc. due to diffraction of X rays by different atoms of the substance.

Binding of specific proteins on regulatory sequences of DNA will generate specific diffraction patterns of X rays which can help to identify specific proteins binding to specific regulatory regions in DNA.

Hence, D is the correct option.