The correct option is
D Avena curvature test
Bioassay is a sensitive and specific test that determines the biological activity of hormones by measuring its effect on a specific part of plant material. There are various bioassays to determine the concentration of auxin hormone in plant such as Avena coleoptile test, split pea stem curvature test, cress root inhibition test. The Avena coleoptile curvature test is a bioassay for IAA (indole-3-acetic acid) that is dependent on differential growth due to the polar transport of IAA from the tip towards the base of coleoptiles in Avena. So, the correct answer is 'Avena curvature test'