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DNA Fingerprinting

blotting meth...

Question

__ blotting method is used in DNA fingerprinting.

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Solution

Edwin Southern developed the Southern blotting method to detect specific sequences in DNA samples. In this method, DNA is broken down into fragments, STRs are identified in them and amplified. They are then separated by running on gel electrophoresis.

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Q. DNA fingerprinting method is very useful for

Q. Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.

A

. Electrophoresis of DNA fragments

B

. Hybridisation with DNA probe

C

. Digestion of DNA by RENs

D

. Autoradiography

E

. Blotting of DNA fragments to nitrocellulose membrane

Q. Arrange in a sequence the given protocol for DNA fingerprinting:
(a) Denaturating DNA

(c) Extraction of DNA

(d) Fragmenting DNA

(e) Separation of DNA fragments by electrophoresis

(f) Hybridisation with the probe

(g) Exposure on film to make a DNA fingerprint

Q. Select the correct sequences of steps in DNA fingerprinting involving southern blot hybridization using radiolabelled VNTR as probe.
I. Hybridisation using labelled VNTR probe
II. Isolation of DNA
III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
IV. Detection of hybridized DNA fragments by autoradiography
V. Separation of DNA fragments by electrophoresis
VI. Digestion of DNA by restriction endonucleases

Q. The very first method used for DNA fingerprinting is

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