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Photosynthesis Experiments

Bottles conta...

Question

Bottles containing the undermentioned liquids were available in the laboratory. Which liquid did a student use for putting a drop on the slide before placing the coverslip while preparing the temporary mount of leaf epidermal peel?
(1) Water
(2) Safranin
(3) Glycerine
(4) Iodine

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Solution

The student must have put a drop of glycerine on the slide before placing the coverslip on the slide. Glycerine is a viscous liquid which prevents the specimen from drying out and keeps it moist for longer duration. Also glycerine tends to refract light due to its refractive nature. As a result of which, the image appears clearer under the microscope.

Hence, the correct answer is option 3.

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Q. The temporary mount of the leaf epidermal peel which looked pinkish red under the microscope was :
(1) stained in acetocarmine and mounted in glycerine
(2) stained in iodine and mounted in water
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(4) stained in methylene blue and mounted in water

Q. While preparing a good temporary mount of leaf peel to observe stomata, care should be taken to avoid:
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Q. In an experiment to prepare temporary mount of a leaf peel staining of leaf peel is done before putting a drop of glycerine. Why?

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leaf peel on slide.
(I) to put a single drop of stain on leaf peel and wash it with water.
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(IV) observe the slide under microscope.
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The correct sequence of the experiment steps is

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