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Question

Bottles containing the undermentioned liquids were available in the laboratory. Which liquid did a student use for putting a drop on the slide before placing the coverslip while preparing the temporary mount of leaf epidermal peel?
(1) Water
(2) Safranin
(3) Glycerine
(4) Iodine

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Solution

The student must have put a drop of glycerine on the slide before placing the coverslip on the slide. Glycerine is a viscous liquid which prevents the specimen from drying out and keeps it moist for longer duration. Also glycerine tends to refract light due to its refractive nature. As a result of which, the image appears clearer under the microscope.

Hence, the correct answer is option 3.

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