Question

Callus formation is promoted by

• A. Proper light and subculturing
• B. Darkness and subculturing
• C. Excess of NAA
• D. Absence of salts

Answer 1

The correct option is B Darkness and subculturing

Callus is unorganized actively dividing the mass of cells maintained in an artificial culture. It has a meristematic cell which has ample amount of cytoplasm to trigger cell division. It is treated with the growth hormones to allow the cell division and differentiation.

Callus formation is promoted by darkness. Due to the darkness, the callus is unable to perform photosynthesis so it avoids stocking up of phenolics produced by the plants. Subculturing involves the transferring of few cells from the parent culture to the new fresh media. It helps to increase the growth of callus by speeding up cell division.

So, the correct answer is option B.