Question

Calvin isolated the different chemicals of Calvin cycle by

• A. Autoradiography
• B. Chromatography
• C. Cell fractionation
• D. X-ray diffraction

Answer 1

The correct option is B Chromatography

Autoradiography includes labelling of biomolecules with different radioactive materials (radioactive thymine for DNA, radioactive uracil for RNA and radioactive S for proteins) followed by localizing these radioactively labelled molecules by applying photosensitive emulsion on the surface of prepared slides. Calvin added trace amounts of radioactive carbon to the illuminated suspension of Chlorella and stopped its growth at various stages and isolated the intermediates of Calvin cycle using paper chromatography, a technique of isolation of chemicals of a solution using the advantage of differential migration rates along the paper. Cellular fractionation techniques refer to the separation of all cellular organelles through the sequential homogenization, filtration and purification methods. X-ray diffraction identifies the atomic and molecular structure of a crystal by the differential pattern of diffraction of incident X-rays by crystalline atoms cause

So, the correct answer is 'Chromatography'