Question

Can a denatured enzyme be renatured?

Answer 1

Enzyme denaturation:

1. Enzyme denaturation is the process in which the conformational change of protein structure occurs in response to a denaturing agent. The result is the active site's conformation is altered and the activity of the enzyme is lost.
2. No, a denatured enzyme cannot be renatured.
3. The reason is that during denaturation the bonds in the enzymes are broken so the structure gets disrupted. Renaturation is possible in those cases only when the primary structure of the protein is intact If the covalent bonds holding the correct sequence of amino acids remain intact then renaturation is possible.
4. In the case of the enzyme, as the conformation of the active site gets changed so the enzyme cannot be renatured.
5. So, a denatured enzyme cannot be renatured.