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Question

cDNA is formed by

A
DNA dependent DNA polymerase
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B
RNA dependent DNA polymerase
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C
DNA dependent RNA polymerase
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D
DNA ligase
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Solution

The correct option is A RNA dependent DNA polymerase
The DNA synthesized using RNA template is known as cDNA or complementary DNA. DNA synthesis is carried out by DNA polymerase and not by RNA polymerase. RNA retroviruses have reverse transcriptase enzyme that synthesizes a DNA copy (cDNA) of viral RNA. The enzymes uses RNA as the template to synthesize DNA, hence the name RNA-dependent DNA polymerase. DNA ligase enzyme joins two DNA fragments together via phosphodiester bond between 3’ hydroxyl at the end of one fragment and a 5’ phosphate at the end of another fragment; it does not polymerise DNA. The correct answer is B.

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