Question

cDNA probes are copied from the messenger RNA molecules with the help of which of the following enzymes?

• A. Restriction enzymes
• B. Reverse transcriptase
• C. DNA polymerase
• D. Adenosine deaminase

Answer 1

The correct option is B Reverse transcriptase

A. Restriction enzymes are the endonucleases that make a cut at specific positions within the DNA.

B. Reverse transcriptase is the enzyme that synthesise a complementary DNA by using RNA strand as template.

C. DNA polymerase is the enzyme that synthesise new DNA strand from DNA template.

D. Adenosine deaminase breakdowns adenosine into inosine.

Hence, cDNA probes are copied from the messenger RNA molecules with the help of Reverse transcriptase. So, the correct answer is 'Reverse transcriptase'.