The correct option is D Double stranded DNA
Chargaff's rule states that the ratios of A to T and G to C bases are approximately equal in a DNA helix. This is a result of complementary base pairing between single strands of DNA in a helix. Each A pairs with a T and each G pairs with a C, making their molar ratios equal. Hence it is only applicable for dsDNA.
RNA contains the bases - Adenine, guanine, uracil and cytosine.